Program Abstracts - Reut Shalgi, Rappaport Faculty of Medicine, Technion, Israel

Systems biology, genomics and proteomics. Dynamic Regulation of Chaperone Interaction Networks

Reut Shalgi, Technion

Protein homeostasis, the balance between protein synthesis, folding, localization and degradation, is a core principal that living organisms strive to maintain. Cells and organisms live in a continuously changing environment, and are required to constantly respond and adapt to various environmental cues. Thus, cells have evolved complex mechanisms to control protein homeostasis, at the hearts of which are the master regulators of protein folding and aggregation, molecular chaperones. We recently discovered that, in mammalian cells, dynamic association of chaperones with the ribosome serves to mediate translational control in response to proteotoxic stress. Using high-throughput LUMIER protein-protein interaction assays, we map chaperone interaction networks and explore their dynamics in response to changing cellular environments, and during disease states. We are generating and combining transcriptome-wide expression and translation data, with protein-protein interaction networks, towards the goal of gaining new insights into regulation of protein homeostasis under challenging environments. 

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